Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Identifieur interne : 001163 ( Main/Exploration ); précédent : 001162; suivant : 001164Prokaryotic expression and characterization of avian influenza A virus M2 gene as a candidate for universal recombinant vaccine against influenza A subtypes; specially H5N1 and H9N2
Auteurs : Seyyed Mahmoud Ebrahimi [Iran] ; Majid Tebianian [Iran] ; Khosrow Aghaiypour [Iran] ; Hassan Nili [Iran] ; Ali Mirjalili [Iran]Source :
- Molecular Biology Reports [ 0301-4851 ] ; 2010-07-01.
Descripteurs français
- Wicri :
- topic : Grippe aviaire.
English descriptors
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Abstract
Abstract: The conserved M2 protein of influenza A virus is considered as a promising candidate target for a broad-spectrum, recombinant influenza A vaccine. In the present study, the open reading frame (ORF) of avian influenza A/chicken/Iran/101/1998 (H9N2) M2 gene was amplified then cloned in pAED4, prokaryotic expression vector. M2 protein was produced through the expression of this recombinant expression vector (pAED4-M2) in E. coli BL21 (DE3) strain. The expressed M2 protein was analyzed on SDS-PAGE. Western blot assay was used to examine the immunoreaction of the expressed protein using commercial polyclonal anti-M2 antibody. The antigenicity and biological activity of the recombinant protein was also qualitatively detected on infected MDCK cells surface by immunofluorescence assay using rabbit’s immunized antiserum. So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.
Url:
DOI: 10.1007/s11033-009-9851-5
Affiliations:
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So, according to the sequence alignment based on the mentioned isolate and the result of immunoassay reaction, it seems recombinant vaccine based on A/chicken/Iran/101/1998(H9N2) M2 protein isolate might cover majority of influenza A virus strains specially H5 and H9 circulating in Iran and neighbor regions significantly.</div></front></TEI><affiliations><list><country><li>Iran</li></country></list><tree><country name="Iran"><noRegion><name sortKey="Ebrahimi, Seyyed Mahmoud" sort="Ebrahimi, Seyyed Mahmoud" uniqKey="Ebrahimi S" first="Seyyed Mahmoud" last="Ebrahimi">Seyyed Mahmoud Ebrahimi</name></noRegion><name sortKey="Aghaiypour, Khosrow" sort="Aghaiypour, Khosrow" uniqKey="Aghaiypour K" first="Khosrow" last="Aghaiypour">Khosrow Aghaiypour</name><name sortKey="Ebrahimi, Seyyed Mahmoud" sort="Ebrahimi, Seyyed Mahmoud" uniqKey="Ebrahimi S" first="Seyyed Mahmoud" last="Ebrahimi">Seyyed Mahmoud Ebrahimi</name><name sortKey="Ebrahimi, Seyyed Mahmoud" sort="Ebrahimi, Seyyed Mahmoud" uniqKey="Ebrahimi S" first="Seyyed Mahmoud" last="Ebrahimi">Seyyed Mahmoud Ebrahimi</name><name sortKey="Mirjalili, Ali" sort="Mirjalili, Ali" uniqKey="Mirjalili A" first="Ali" last="Mirjalili">Ali Mirjalili</name><name sortKey="Nili, Hassan" sort="Nili, Hassan" uniqKey="Nili H" first="Hassan" last="Nili">Hassan Nili</name><name sortKey="Tebianian, Majid" sort="Tebianian, Majid" uniqKey="Tebianian M" first="Majid" last="Tebianian">Majid Tebianian</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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